Composite

Part:BBa_K4040024:Design

Designed by: Tong Yin   Group: iGEM21_NMU_China   (2021-09-27)


pTET-mCherry-PGK-CD20 Expression Cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1649
    Illegal PstI site found at 2560
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1649
    Illegal PstI site found at 2560
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1649
    Illegal PstI site found at 2560
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1649
    Illegal PstI site found at 2560
    Illegal NgoMIV site found at 2407
    Illegal AgeI site found at 1115
    Illegal AgeI site found at 1227
    Illegal AgeI site found at 1514
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2654
    Illegal BsaI.rc site found at 2723
    Illegal BsaI.rc site found at 2729


Design Notes

N


Source

N

References